Publication list - Peer reviewed publications

Peer reviewed publications:
Research investigations
2020
Fujioka H, Shou J, Kojima R, Urano Y, Ozeki Y, Kamiya M: Multicolor Activatable Raman Probes for Simultaneous Detection of Plural Enzyme Activities. J. Am. Chem. Soc. 2020. doi: doi.org/10.1021/jacs.0c09200.
Fujita K, Kamiya M, Yoshioka T, Ogasawara A, Hino R, Kojima R, Ueo H, Urano Y: Rapid and Accurate Visualization of Breast Tumors with a Fluorescent Probe Targeting α-Mannosidase 2C1. ACS Cent. Sci. 2020. doi: 10.1021/acscentsci.0c01189.
Obara R, Kamiya M, Tanaka Y, Abe A, Kojima R, Kawaguchi T, Sugawara M, Takahashi A, Noda T, Urano Y: γ‐Glutamyltranspeptidase (GGT)‐Activatable Fluorescence Probe for Durable Tumor Imaging. Angew Chem Int Ed Engl. 2020. doi: 10.1002/anie.202013265.
Akashi T, Isomoto H, Matsushima K, Kamiya M, Kanda T, Nakano M, Onoyama T, Fujii M, Akada J, Akazawa Y, Ohnita K, Takeshima F, Nakao K, Urano Y: A novel method for rapid detection of a Helicobacter pylori infection using a γ-glutamyltranspeptidase-activatable fluorescent probe. Sci Rep. 2019 9(1):9467. doi: 10.1038/s41598-019-45768-x.
Yamamoto K, Ohnishi S, Mizushima T, Kodaira J, Ono M, Hatanaka Y, Hatanaka KC, Kuriki Y, Kamiya M, Ehira N, Shinada K, Takahashi H, Shimizu Y, Urano Y, Sakamoto N: Detection of early adenocarcinoma of the esophagogastric junction by spraying an enzyme-activatable fluorescent probe targeting Dipeptidyl peptidase-IV. BMC Cancer. 2020 20(1):64. doi: 10.1186/s12885-020-6537-9.
Kawashima S, Yoshioka T, Hino H, Kitano K, Nagayama K, Sato M, Kojima R, Kamiya M, Urano Y, Nakajima J: γ-glutamyl hydroxymethyl rhodamine green fluorescence as a prognostic indicator for lung cancer. Gen Thorac Cardiovasc Surg. 2020. doi: 10.1007/s11748-020-01395-2.
Asano T, Nagayo Y, Tsuda S, Ito A, Kobayashi W,Fujita K, Sato K, Nishiguchi KM, Kunikata H, Fujioka H, Kamiya M, Urano Y, Nakazawa T: Companion Diagnosis for Retinal Neuroprotective Treatment by Real-Time Imaging of Calpain Activation Using a Novel Fluorescent Probe Bioconjug Chem. 2020 31(9):2241-2251. doi: 10.1021/acs.bioconjchem.0c00435.
Kono Y, Ishizawa T, Kokudo N, Kuriki Y, Iwatate JR, Kamiya M, Urano Y, Kumagai A, Kurokawa H, Miyawaki A, Hasegawa K: On-Site Monitoring of Postoperative Bile Leakage Using Bilirubin-Inducible Fluorescent Protein World J Surg. 2020 doi: 10.1007/s00268-020-05774-x.
Tachibana R, Kamiya M, Suzuki S, Morokuma K, Nanjo A, Urano Y: Molecular design strategy of fluorogenic probes based on quantum chemical prediction of intramolecular spirocyclization. Commun Chem. 2020 3(82). doi: 10.1038/s42004-020-0326-x.
Morozumi A, Kamiya M, Uno SN, Umezawa K, Kojima R, Yoshihara T, Tobita S, Urano Y: Spontaneously Blinking Fluorophores Based on Nucleophilic Addition/Dissociation of Intracellular Glutathione for Live-Cell Super-resolution Imaging. J. Am. Chem. Soc. 2020. doi: 10.1021/jacs.0c00451
Fujioka H, Uno SN, Kamiya M, Kojima R, Johnsson K, Urano Y: Activatable fluorescent probes for hydrolase enzymes based on coumarin-hemicyanine hybrid fluorophores with large Stokes shifts. Chem Commun (Camb). 2020. doi: 10.1039/d0cc00559b.
2019
Rivas C, Kamiya M, Urano Y: A novel sialidase-activatable fluorescence probe with improved stability for the sensitive detection of sialidase. Bioorg Med Chem Lett. 2019 126860. doi: 10.1016/j.bmcl.2019.126860.
Akashi T, Isomoto H, Matsushima K, Kamiya M, Kanda T, Nakano M, Onoyama T, Fujii M, Akada J, Akazawa Y, Ohnita K, Takeshima F, Nakao K, Urano Y: A novel method for rapid detection of a Helicobacter pylori infection using a γ-glutamyltranspeptidase-activatable fluorescent probe. Sci Rep. 2019 9(1):9467. doi: 10.1038/s41598-019-45768-x.
Kitagawa Y, Tanaka S, Kuriki Y, Yamamoto K, Ogasawara A, Nejo T, Matsuura R, Koike T, Hana T, Takahashi S, Nomura M, Takayanagi S, Mukasa A, Kamiya M, Urano Y, Saito N: Spray Fluorescent Probes for Fluorescence-Guided Neurosurgery. Front Oncol. 2019 9:727. doi: 10.3389/fonc.2019.00727. eCollection 2019.
Yamamoto K, Kamiya M, Urano Y: Highly sensitive fluorescence imaging of cancer with avidin-protease probe conjugate. Bioorg Med Chem Lett. 2019 29(20):126663. doi: 10.1016/j.bmcl.2019.126663.
Chiba M, Kamiya M, Tsuda S K, Fujisawa Y, Kosakamoto H, Kojima R, Miura M, Urano Y: Activatable Photosensitizer for Targeted Ablation of lacZ-Positive Cells with Single-Cell Resolution. ACS Cent. Sci. 2019. doi: 10.1021/acscentsci.9b00678
Kawatani M, Yamamoto K, Yamada D, Kamiya M, Miyakawa J, Miyama Y, Kojima R, Morikawa T, Kume H1, Urano Y: Fluorescence Detection of Prostate Cancer by an Activatable Fluorescence Probe for PSMA Carboxypeptidase Activity. J Am Chem Soc. 2019 141(26):10409-10416. doi: 10.1021/jacs.9b04412.
Ogasawara A, Kamiya M, Sakamoto K, Kuriki Y, Fujita K, Komatsu T, Ueno T, Hanaoka K, Onoyama H, Abe H, Tsuji Y, Fujishiro M, Koike K, Fukayama M, Seto Y, Urano Y: Red Fluorescence Probe Targeted to Dipeptidylpeptidase-IV for Highly Sensitive Detection of Esophageal Cancer. Bioconjug Chem. 2019 30(4):1055-1060. doi: 10.1021/acs.bioconjchem.9b00198.
Inagaki Y, Kokudo T, Kamiya M, Uno SN, Sato M, Kaneko J, Kokudo N, Urano Y, Hasegawa K4: A novel liver-specific fluorescent anti-cancer drug delivery system using indocyanine green. Sci Rep. 2019 9(1):3044. doi: 10.1038/s41598-019-39269-0.
2018
Kubo H, Hanaoka K, Kuriki Y, Komatsu T, Ueno T, Kojima R, Kamiya M, Murayama Y, Otsuji E, Urano Y: Rapid detection of metastatic lymph nodes of colorectal cancer with a gamma-glutamyl transpeptidase-activatable fluorescence probe. Sci Rep. 2018 8(1):17781. doi: 10.1038/s41598-018-36062-3.
Hino R, Inoshita N, Yoshimoto T, Ogawa M, Miura D, Watanabe R, Watanabe K, Kamiya M, Urano Y: Rapid detection of papillary thyroid carcinoma by fluorescence imaging using a γ-glutamyltranspeptidase-specific probe: a pilot study. Thyroid Res. 2018 11:16. doi: 10.1186/s13044-018-0060-y. eCollection 2018.
Moriizumi Y, Tabata KV, Watanabe R, Doura T, Kamiya M, Urano Y, Noji H: Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device. Sci Rep. 2018 8(1):11757. doi: 10.1038/s41598-018-30231-0.
Ida S, Hiki N, Ishizawa T, Kuriki Y, Kamiya M, Urano Y, Nakamura T, Tsuda Y, Kano Y, Kumagai K, Nunobe S, Ohashi M, Sano T: Pancreatic Compression during Lymph Node Dissection in Laparoscopic Gastrectomy: Possible Cause of Pancreatic Leakage. J Gastric Cancer. 2018 18(2):134-141. doi: 10.5230/jgc.2018.18.e15.
Tabe H, Sukenobe K, Kondo T, Sakurai A, Maruo M, Shimauchi A, Hirano M, Uno SN, Kamiya M, Urano Y, Matsushita M, Fujiyoshi S: Cryogenic Fluorescence Localization Microscopy of Spectrally Selected Individual FRET Pairs in a Water Matrix. J Phys Chem B. 2018 Jul 12;122(27):6906-6911. doi: 10.1021/acs.jpcb.8b03977.
Hirasawa T, Iwatate RJ, Kamiya M, Okawa S, Fujita M, Urano Y, Ishihara M: Spectral-differential-based unmixing for multispectral photoacoustic imaging. Appl Opt. 2018 57(10):2383-2393. doi: 10.1364/AO.57.002383.
Ito H, Kawamata Y, Kamiya M, Tsuda-Sakurai K, Tanaka S, Ueno T, Komatsu T, Hanaoka K, Okabe S, Miura M, Urano Y: Red-shifted fluorogenic substrate for detecting lacZ‐positive cells in living tissue with single-cell resolution. Angew. Chem. Int. Ed. 2018 doi: doi.org/10.1002/anie.201808670.
Umezawa K, Kamiya M, Urano Y: A reversible fluorescent probe for real-time live-cell imaging and quantification of endogenous hydropolysulfides. Angew Chem Int Ed Engl. 2018 doi: 10.1002/anie.201804309.
Mizushima T, Ohnishi S, Shimizu Y, Hatanaka Y, Hatanaka KC, Kuriki Y, Kamiya M, Homma A, Yamamoto K, Ono S, Urano Y, Sakamoto N: Rapid detection of superficial head and neck squamous cell carcinoma by topically spraying fluorescent probe targeting dipeptidyl peptidase-IV. Head Neck. 2018 doi: 10.1002/hed.25126.
Kuriki Y, Kamiya M, Kubo H, Komatsu T, Ueno T, Tachibana R, Hayashi K, Hanaoka K, Yamashita S, Ishizawa T, Kokudo N, Urano Y: Establishment of Molecular Design Strategy To Obtain Activatable Fluorescent Probes for Carboxypeptidases. J Am Chem Soc. 2018 140(5):1767-1773. doi: 10.1021/jacs.7b11014.
Okubo K, Kurosawa M, Kamiya M, Urano Y, Suzuki A, Yamamoto K, Hase K, Homma K, Sasaki J, Miyauchi H, Hoshino T, Hayashi M, Mayadas TN, Hirahashi J: Macrophage extracellular trap formation promoted by platelet activation is a key mediator of rhabdomyolysis-induced acute kidney injury. Nat Med. 2018 24(2):232-238. doi: 10.1038/nm.4462.
Shiratori T, Kyumoto-Nakamura Y, Kukita A, Uehara N, Zhang J, Koda K, Kamiya M, Badawy T, Tomoda E, Xu X, Yamaza T, Urano Y, Koyano K, Kukita T: IL-1β Induces Pathologically Activated Osteoclasts Bearing Extremely High Levels of Resorbing Activity: A Possible Pathological Subpopulation of Osteoclasts, Accompanied by Suppressed Expression of Kindlin-3 and Talin-1. J Immunol. 2018 200(1):218-228. doi: 10.4049/jimmunol.1602035.
Iwatate RJ, Kamiya M, Umezawa K, Kashima H, Nakadate M, Kojima R, Urano Y: Silicon Rhodamine-Based Near-Infrared Fluorescent Probe for γ-Glutamyltransferase. Bioconjug Chem. 2018 29(2):241-244. doi: 10.1021/acs.bioconjchem.7b00776.
Uno S, Kamiya M, Morozumi A, Urano Y: A green-light-emitting, spontaneously blinking fluorophore based on intramolecular spirocyclization for dual-colour super-resolution imaging. Chemical Communications. 2018 54:102-105. doi: 10.1039/C7CC07783A.
Kawatani M, Kamiya M, Takahashi H, Urano Y: Factors affecting the uncaging efficiency of 500nm light-activatable BODIPY caging group. Bioorganic & Medicinal Chemistry Letters. 2018 28(1):1-5. doi: 10.1016/j.bmcl.2017.11.030.
2017
Yogo T, Umezawa K, Kamiya M, Hino R, Urano Y: Development of an Activatable Fluorescent Probe for Prostate Cancer Imaging. Bioconjug Chem. 2017 28(8):2069-2076. doi: 10.1021/acs.bioconjchem.7b00233.
Miyata Y, Ishizawa T, Kamiya M, Yamashita S, Hasegawa K, Ushiku A, Shibahara J, Fukayama M, Urano Y, Kokudo N: Intraoperative imaging of hepatic cancers using γ-glutamyltranspeptidase-specific fluorophore enabling real-time identification and estimation of recurrence. Sci Rep. 2017 7(1):3542. doi: 10.1038/s41598-017-03760-3.
Chiba M, Ichikawa Y, Kamiya M, Komatsu T, Ueno T, Hanaoka K, Nagano T, Lange N, Urano Y: An Activatable Photosensitizer Targeted to γ-Glutamyltranspeptidase. Angew Chem Int Ed Engl. 2017 56(35):10418-10422. doi: 10.1002/anie.201704793.
Takakura H, Zhang Y, Erdmann SR, Thompson DA, Lin Y, McNellis B, Rivera-Molina F, Uno S, Kamiya M, Urano Y, Rothman EJ, Bewersdorf J, Schepartz A, Toomre D: Long time-lapse nanoscopy with spontaneously blinking membrane probes. Nature Biotechnology. 2017 35:773-780. doi: 10.1038/nbt.3876.
Shimane T, Aizawa H, Koike T, Kamiya M, Urano Y, Kurita H: Oral cancer intraoperative detection by topically spraying a γ-glutamyl transpeptidase-activated fluorescent probe. Oral Oncol. 2016 54:16-8. doi: 10.1016/j.oraloncology.2015.12.003.
Onagi J, Komatsu T, Ichihashi Y, Kuriki Y, Kamiya M, Terai T, Ueno T, Hanaoka K, Matsuzaki H, Hata K, Watanabe T, Nagano T, Urano Y: Discovery of Cell-Type-Specific and Disease-Related Enzymatic Activity Changes via Global Evaluation of Peptide Metabolism. J Am Chem Soc. 2017 139(9):3465-3472. doi: 10.1021/jacs.6b11376.
2016
Hirasawa T, Iwatate JR, Kamiya M, Okawa S, Urano Y, Ishihara M: Multispectral photoacoustic imaging of tumours in mice injected with an enzyme-activatable photoacoustic probe. Journal of Optics 2017 19(1). doi: 10.1088/2040-8978/19/1/014002.
Umezawa K, Yoshida M, Kamiya M, Yamasoba T, Urano Y: Rational design of reversible fluorescent probes for live-cell imaging and quantification of fast glutathione dynamics. NATURE CHEMISTRY. 2016 9(3):279-286. doi: 10.1038/nchem.2648.
Mizushima T, Ohnishi S, Shimizu Y, Hatanaka Y, Hatanaka KC, Hosono H1, Kubota Y, Natsuizaka M, Kamiya M, Ono S, Homma A, Kato M, Sakamoto N, Urano Y: Fluorescent imaging of superficial head and neck squamous cell carcinoma using a γ-glutamyltranspeptidase-activated targeting agent: a pilot study. BMC Cancer. 2016 16:411. doi: 10.1186/s12885-016-2421-z.
Doura T, Kamiya M, Obata F, Yamaguchi Y, Hiyama TY, Matsuda T, Fukamizu A, Noda M, Miura M, Urano Y: Detection of LacZ-Positive Cells in Living Tissue with Single-Cell Resolution. Angew Chem Int Ed Engl. 2016 55(33):9620-4. doi: 10.1002/anie.201603328.
Okubo K, Kamiya M, Urano Y, Nishi H, Herter JM, Mayadas T, Hirohama D, Suzuki K, Kawakami H, Tanaka M, Kurosawa M, Kagaya S, Hishikawa K, Nangaku M, Fujita T, Hayashi M, Hirahashi J: Lactoferrin Suppresses Neutrophil Extracellular Traps Release in Inflammation. EBioMedicine. 2016 10:204-15. doi: 10.1016/j.ebiom.2016.07.012.
Shinden Y, Ueo H, Tobo T, Gamachi A, Utou M, Komatsu H, Nambara S, Saito T, Ueda M, Hirata H, Sakimura S, Takano Y, Uchi R, Kurashige J, Akiyoshi S, Iguchi T, Eguchi H, Sugimachi K, Kubota Y, Kai Y, Shibuta K, Kijima Y, Yoshinaka H, Natsugoe S, Mori M, Maehara Y, Sakabe M, Kamiya M, Kakareka JW, Pohida TJ, Choyke PL, Kobayashi H, Ueo H, Urano Y, Mimori K: Rapid diagnosis of lymph node metastasis in breast cancer using a new fluorescent method with γ-glutamyl hydroxymethyl rhodamine green. Sci Rep. 2016 6:27525. doi: 10.1038/srep27525.
Onoyama H, Kamiya M, Kuriki Y, Komatsu T, Abe H, Tsuji Y, Yagi K, Yamagata Y, Aikou S, Nishida M, Mori K, Yamashita H, Fujishiro M, Nomura S, Shimizu N, Fukayama M, Koike K, Urano Y, Seto Y: Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV. Sci Rep. 2016 6:26399. doi: 10.1038/srep26399.
Hino H, Kamiya M, Kitano K, Mizuno K, Tanaka S, Nishiyama N, Kataoka K, Urano Y, Nakajima J: Rapid Cancer Fluorescence Imaging Using A γ-Glutamyltranspeptidase-Specific Probe For Primary Lung Cancer. Translational Oncology. 2016 9(3):203-210. doi: 10.1016/j.tranon.2016.03.007.
Matsuzaki H, Kamiya M, Iwatate RJ, Asanuma D, Watanabe T, Urano Y: Novel Hexosaminidase-Targeting Fluorescence Probe for Visualizing Human Colorectal Cancer. Bioconjug Chem. 2016. 2016 27(4):973-81. doi: 10.1021/acs.bioconjchem.6b00037.
Anraku Y, Kishimura A, Kamiya M, Tanaka S, Nomoto T, Toh K, Matsumoto Y, Fukushima S, Sueyoshi D, Kano MR, Urano Y, Nishiyama N, Kataoka K: Systemically Injectable Enzyme-Loaded Polyion Complex Vesicles as In Vivo Nanoreactors Functioning in Tumors. Angew Chem Int Ed Engl. 2016 55(2):560-5. doi: 10.1002/anie.201508339.
2015
Iwatate RJ, Kamiya M, Urano Y: Asymmetric Rhodamine-Based Fluorescence Probes for Multi-colour In Vivo Imaging. Chemistry. 2015 22(5):1696-703. doi: 10.1002/chem.201503426
Hirakawa Y, Yoshihara T, Kamiya M, Mimura I, Fujikura D, Masuda T, Kikuchi R, Takahashi I, Urano Y, Tobita S, Nangaku M: Quantitating intracellular oxygen tension in vivo by phosphorescence lifetime measurement. Sci Rep. 2015 5:17838. doi: 10.1038/srep17838.
Kojima R, Takakura H, Kamiya M, Kobayashi E, Komatsu T, Ueno T, Terai T, Hanaoka K, Nagano T, Urano Y: Development of a Sensitive Bioluminogenic Probe for Imaging Highly Reactive Oxygen Species in Living Rats. Angew Chem Int Ed Engl. 2015 54(49):14768-71. doi: 10.1002/anie.201507530.
Hirabayashi K, Hanaoka K, Takayanagi T, Toki Y, Egawa T, Kamiya M, Komatsu T, Ueno T, Terai T, Yoshida K, Uchiyama M, Nagano T, Urano Y: Analysis of Chemical Equilibrium of Silicon-Substituted Fluorescein and Its Application to Develop a Scaffold for Red Fluorescent Probes. Anal Chem. 2015 87(17):9061-9. doi: 10.1021/acs.analchem.5b02331.
Hayashi A, Asanuma D, Kamiya M, Urano Y, Okabe S: High affinity receptor labeling based on basic leucine zipper domain peptides conjugated with pH-sensitive fluorescent dye: Visualization of AMPA-type glutamate receptor endocytosis in living neurons. Neuropharmacology. 2015 100:66-75. doi: 10.1016/j.neuropharm.2015.07.026.
Ueo H, Shinden Y, Tobo T, Gamachi A, Udo M, Komatsu H, Nambara S, Saito T, Ueda M, Hirata H, Sakimura S, Takano Y, Uchi R, Kurashige J, Akiyoshi S, Iguchi T, Eguchi H, Sugimachi K, Kubota Y, Kai Y, Shibuta K, Kijima Y, Yoshinaka H, Natsugoe S, Mori M, Maehara Y, Sakabe M, Kamiya M, Kakareka JW, Pohida TJ, Choyke PL, Kobayashi H, Ueo H, Urano Y, Mimori K: Rapid intraoperative visualization of breast lesions with γ-glutamyl hydroxymethyl rhodamine green. Sci Rep. 2015 5:12080. doi: 10.1038/srep12080.
Uno S, D.K.Tiwari, Kamiya M, Arai Y, Nagai T, Urano Y: A guide to use photocontrollable fluorescent proteins and synthetic smart fluorophores for nanoscopy. Microscopy (Oxf). 2015 64(4):263-77. doi: 10.1093/jmicro/dfv037.
Asanuma D, Sakabe M, Kamiya M, Yamamoto K, Hiratake J, Ogawa M, Kosaka N, Choyke PL, Nagano T, Kobayashi H, Urano Y: Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo. Nat Commun. 2015 6:6463. doi: 10.1038/ncomms7463.
Takakura H, Kojima R, Kamiya M, Kobayashi E, Komatsu T, Ueno T, Terai T, Hanaoka K, Nagano T, Urano Y: New Class of Bioluminogenic Probe Based on Bioluminescent Enzyme-Induced Electron Transfer: BioLeT. J. Am. Chem. Soc. 2015 137(12):4010-3. doi: 10.1021/ja511014w.
Sato C, Abe S, Saito Y, Tsuruki E S, Takamaru H, Makazu M, Sato Y, Sasaki H, Tanaka H, Ikezawa N, Yamada M, Sakamoto T, Nakajima T, Matsuda T, Kushima R, Kamiya M, Maeda S, Urano Y: A Pilot Study of Fluorescent Imaging of Colorectal Tumors Using a γ-Glutamyl-Transpeptidase-Activatable Fluorescent Probe. Digestion. 2015 91(1):70-6. doi: 10.1159/000369367.
2014
Arai H C, Yukawa A, Iwatate R J, Kamiya M, Watanabe R, Urano Y, Noji H: Torque Generation Mechanism of F1-ATPase upon NTP Binding. Biophysical Journal 2014 107(1):156-64. doi: 10.1016/j.bpj.2014.05.016.
Fujii T, Kamiya M, Urano Y: In Vivo Imaging of Intraperitoneally Disseminated Tumors in Model Mice by Using Activatable Fluorescent Small-Molecular Probes for Activity of Cathepsins. Bioconjug Chem. 2014 25(10):1838-46. doi: 10.1021/bc5003289.
Umeda N, Takahashi H, Kamiya M, Ueno T, Komatsu T, Terai T, Hanaoka K, Nagano T, Urano Y: Boron dipyrromethene as a fluorescent caging group for single-photon uncaging with long-wavelength visible light. ACS Chemical Biology. 2014 9(10):2242-6. doi: 10.1021/cb500525p.
Yoshida M, Kamiya M, Yamasoba T, Urano Y: A highly sensitive, cell-membrane-permeable fluorescent probe for glutathione. Bioorg Med Chem Lett. 2014 24(18):4363-6. doi: 10.1016/j.bmcl.2014.08.033.
Watanabe R, Soga N, Fujita D, Tabata VK, Yamauchi L, Kim HS, Asanuma D,Kamiya M, Urano Y, Suga H, Noji H: Arrayed lipid bilayer chambers allow single-molecule analysis of membrane transporter activity. Nat Commun. 2014 5(4519). doi: 10.1038/ncomms5519.
Uno S, Kamiya M, Yoshihara T, Sugawara K, Okabe K, Tarhan MC, Fujita H, Funatsu T, Okada Y, Tobita S, Urano Y: A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging. Nature Chemistry. 2014 6:681-9. doi: 10.1038/nchem.2002.
Ishizawa T, Masuda K, Urano Y, Kawaguchi Y, Satou S, Kaneko J, Hasegawa K, Shibahara J, Fukayama M, Tsuji S, Midorikawa Y, Aburatani H, Kokudo N: Mechanistic background and clinical applications of indocyanine green fluorescence imaging of hepatocellular carcinoma. Ann Surg Oncol. 2014 21(2):440-8. doi: 10.1245/s10434-013-3360-4.
Asanuma D, Takaoka Y, Namiki S, Takikawa K, Kamiya M, Nagano T, Urano Y, Hirose K: Acidic-pH-activatable Fluorescence Probes for Visualizing Exocytosis Dynamics. Angew Chem. 2014 53(24):6085-9. doi: 10.1002/anie.201402030
Ichikawa Y, Kamiya M, Obata F, Miura M, Terai T, Komatsu T, Ueno T, Hanaoka K, Nagano T, Urano Y: Selective Ablation of β-Galactosidase-expressing Cells with a Rationally Designed Activatable Photosensitizer. Angew Chem. 2014 53(26):6772-6775. doi: 10.1002/anie.201403221.
2013
Yamashita S, Sakabe M, Ishizawa T, Hasegawa K, Urano Y, Kokudo N: Visualization of the leakage of pancreatic juice using a chymotrypsin-activated fluorescent probe.Br J Surg. 2013 100(9):1220-8. doi: 10.1002/bjs.9185.
Terai T, Tomiyasu R, Ota T, Ueno T, Komatsu T, Hanaoka K, Urano Y, Nagano T: TokyoGreen derivatives as specific and practical fluorescent probes for UDP-glucuronosyltransferase (UGT) 1A1.Chem Commun (Camb). 2013 49(30):3101-3. doi: 10.1039/c3cc38810g.
Sakabe M, Asanuma D, Kamiya M, Iwatate JR, Hanaoka K, Terai T, Nagano T, Urano Y: Rational Design of Highly Sensitive Fluorescence Probes For Protease and Glycosidase Based on Precisely Controlled Spirocyclization.J. Am. Chem. Soc. 2013 135(1):409-14. doi: 10.1021/ja309688m.
Mitsunaga M,Kosaka N, Choyke PL, Young MR, Dextras CR, Saud SM, Colburn NH, Sakabe M, Nagano T, Asanuma D, Urano Y, Kobayashi H: Fluorescence endoscopic detection of murine colitis-associated colon cancer by topically applied enzymatically rapid-activatable probe. Gut. 2013 62(8):1179-86. doi: 10.1136/gutjnl-2011-301795.
2012
Urano Y: Novel live imaging techniques of cellular functions and in vivo tumors based on precise design of small molecule-based ‘Activatable’ fluorescence probes. Curr Opin Chem Biol. 2012 16(5-6):602-8. doi: 10.1016/j.cbpa.2012.10.023.
Kojima R, Takakura H, Ozawa T, Tada Y, Nagano T, Urano Y: Rational Design and Development of Near-Infrared-Emitting Firefly Luciferins Available In Vivo. Angew Chem Int Ed Engl. 2013 52(4):1175-9. doi: 10.1002/anie.201205151.
Kobayashi T, Komatsu T, Kamiya M, Campos C, González-Gaitán M, Terai T, Hanaoka K, Nagano T, Urano Y: Highly Activatable and Environment-insensitive Optical Highlighters for Selective Spatiotemporal Imaging of Target Proteins. J Am Chem Soc. 2012 134(27):11153-60. doi: 10.1021/ja212125w.
Takakura H, Kojima R, Ozawa T, Nagano T, Urano Y: Development of 5'- and 7'-Substituted Luciferin Analogues as Acid-Tolerant Substrates of Firefly Luciferase. Chembiochem. 2012 13(10):1424-7. doi: 10.1002/cbic.201200142.
Koide Y, Kawaguchi M, Urano Y, Hanaoka K, Komatsu T, Abo M, Terai T, Nagano T: A reversible near-infrared fluorescence probe for reactive oxygen species based on Te-rhodamine Chem Commun (Camb). 2012 48(25):3091-3. doi: 10.1039/c2cc18011a.
Koide Y, Urano Y, Hanaoka K, Piao W, Kusakabe M, Saito N, Terai T, Okabe T, Nagano T: Development of NIR fluorescent dyes based on Si-rhodamine for in vivo imaging. J Am Chem Soc. 2012 134(11):5029-31. doi: 10.1021/ja210375e.
Terai T, Kikuchi K, Urano Y, Kojima H, Nagano T: A long-lived luminescent probe to sensitively detect arylamine N-acetyltransferase (NAT) activity of cells. Chem Commun (Camb). 2012 48(16):2234-6. doi: 10.1039/c2cc17622j.
Terai T, Urano Y, Izumi S, Kojima H, Nagano T: A practical strategy to create near-infrared luminescent probes: conversion from fluorescein-based sensors. Chem Commun (Camb). 2012 48(23):2840-2. doi: 10.1039/c2cc16553h.
Karaki F, Kabasawa Y, Yanagimoto T, Umeda N, Firman, Urano Y, Nagano T, Otani Y, Ohwada T: Visible-light-triggered release of nitric oxide from N-pyramidal nitrosamines. Chemistry. 2012 18(4):1127-41. doi: 10.1002/chem.201101427.
2011
McCann TE, Kosaka N, Koide Y, Mitsunaga M, Choyke PL, Nagano T, Urano Y, Kobayashi H: Activatable optical imaging with a silica-rhodamine based near infrared (SiR700) fluorophore: a comparison with cyanine based dyes. Bioconjug Chem. 2011 22(12):2531-8. doi: 10.1021/bc2003617.
Urano Y, Sakabe M, Nobuyuki Kosaka, Ogawa M, Mitsunaga M, Asanuma D, Kamiya M, Young MR, Nagano T, Choyke PL, Kobayashi H: Rapid Cancer Detection by Topically Spraying a Gamma-glutamyltranspeptidase-activated Fluorescent Probe. Sci Transl Med. 2011 3(110):110ra119. doi: 10.1126/scitranslmed.3002823.
Kamiya M, Asanuma D, Kuranaga E, Takeishi A, Sakabe M, Miura M, Nagano T, Urano Y: β-Galactosidase Fluorescence Probe with Improved Cellular Accumulation Based on Spirocyclized Rhodol Scaffold. J Am Chem Soc. 2011 133(33):12960-3. doi: 10.1021/ja204781t.
Komatsu T, Oushiki D, Takeda A, Miyamura M, Ueno T, Terai T, Hanaoka K, Urano Y, Mineno T, Nagano T: Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophores applicable to a protein dynamics study. Chem Commun (Camb). 2011 47(36):10055-7. doi: 10.1039/c1cc13367e.
Abo M, Urano Y, Hanaoka K, Terai T, Komatsu T, Nagano T: Development of a highly sensitive fluorescence probe for hydrogen peroxide. J Am Chem Soc. 2011 133(27):10629-37. doi: 10.1021/ja203521e.
Komatsu T, Johnsson K, Okuno H, Bito H, Inoue T, Nagano T, Urano Y: Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method. J Am Chem Soc. 2011 133(17):6745-51. doi: 10.1021/ja200225m.
Koide Y, Urano Y, Hanaoka K, Terai T, Nagano T: Development of an Si-rhodamine-based far-red to near-infrared fluorescence probe selective for hypochlorous acid and its applications for biological imaging. J Am Chem Soc. 2011 133(15):5680-2. doi: 10.1021/ja111470n.
Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T: Aminoluciferins as functional bioluminogenic substrates of firefly luciferase. Chem Asian J. 2011 6(7):1800-10. doi: 10.1002/asia.201000873.
Koide Y, Urano Y, Hanaoka K, Terai T, Nagano T: Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer. ACS Chem Biol. 2011 6(6):600-8. doi: 10.1021/cb1002416.
Matsumoto T, Urano Y, Takahashi Y, Mori Y, Terai T, Nagano T: In situ evaluation of kinetic resolution catalysts for nitroaldol by rationally designed fluorescence probe. J Org Chem. 2011 76(10):3616-25. doi: 10.1021/jo1020344.
Sasaki H, Hanaoka K, Urano Y, Terai T, Nagano T: Design and synthesis of a novel fluorescence probe for Zn2+ based on the spirolactam ring-opening process of rhodamine derivatives. Bioorg Med Chem. 2011 19(3):1072-8. doi: 10.1016/j.bmc.2010.05.074.
2010
Takakura H, Sasakura K, Ueno T, Urano Y, Terai T, Hanaoka K, Tsuboi T, Nagano T: Development of Luciferin Analogues Bearing an Amino Group and Their Application as BRET Donors. Chem Asian J. 2010 5(9):2053-61. doi: 10.1002/asia.201000219.
Yogo T, Urano Y, Kamiya M, Sano K, Nagano T: Development of Enzyme-activated Photosensitizer Based on Intramolecular Electron Transfer. Bioorg Med Chem Lett. 2010 20(15):4320-3. doi: 10.1016/j.bmcl.2010.06.091.
Togashi M, Urano Y, Kojima H, Terai T, Hanaoka K, Igarashi K, Hirata Y, Nagano T: Sensitive Detection of Acrolein in Serum Using Time-resolved Luminescence. Org Lett. 2010 12(8):1704-7. doi: 10.1021/ol1002219.
Kobayashi H, Ogawa M, Alford R, Choyke PL, Urano Y: New Strategies for Fluorescent Probe Design in Medical Diagnostic Imaging. Chem Rev. 2010 110(5):2620-40. doi: 10.1021/cr900263j.
2009
Komatsu T, Urano Y, Fujikawa Y, Kobayashi T, Kojima H, Terai T, Hanaoka K, Nagano T: Development of 2,6-carboxy-substituted boron dipyrromethene (BODIPY) as a novel scaffold of ratiometric fluorescent probes for live cell imaging. Chem Commun (Camb). 2009 (45):7015-7. doi: 10.1039/b917209b.
Izumi S, Urano Y, Hanaoka K, Terai T, Nagano T: A simple and effective strategy to increase the sensitivity of fluorescence probes in living cells. J Am Chem Soc. 2009 131(29):10189-200. doi: 10.1021/ja902511p.
Koide Y, Urano Y, Yatsushige A, Hanaoka K, Terai T, Nagano T: Design and development of enzymatically activatable photosensitizer based on unique characteristics of thiazole orange. J Am Chem Soc. 2009 131(17):6058-9. doi: 10.1021/ja900443b.
Kurashima N, Makino Y, Urano Y, Sanuki K, Ikehara Y, Nagano T: Use of stable isotope ratios for profiling of industrial ephedrine samples: Application of hydrogen isotope ratios in combination with carbon and nitrogen. Forensic Sci Int. 2009 189(1-3):14-8. doi: 10.1016/j.forsciint.2009.04.011.
Kobayashi H, Ogawa M, Kosaka N, Choyke PL, Urano Y: Multicolor imaging of lymphatic function with two nanomaterials: Quantum dot-labeled cancer cells and dendrimer-based optical agents. Nanomedicine (Lond). 2009 4(4):411-9. doi: 10.2217/nnm.09.15.
Urano Y, Asanuma D, Hama Y, Koyama Y, Barrett T, Kamiya M, Nagano T, Watanabe T, Hasegawa A, Choyke PL, Kobayashi H: Selective molecular imaging of viable cancer cells with pH-activatable fluorescence probes. Nat Med. 2009 15(1):104-9. doi: 10.1038/nm.1854.
2008
Matsumoto T, Urano Y, Makino Y, Kikura-Hanajiri R, Kawahara N, Goda Y, Nagano T: Evaluation of characteristic deuterium distributions of ephedrines and methamphetamines by NMR spectroscopy for drug profiling. Anal Chem. 2008 80(4):1176-81. doi: 10.1021/ac701639j.
Fujikawa Y, Urano Y, Komatsu T, Hanaoka K, Kojima H, Terai T, Inoue H, Nagano T: Design and synthesis of highly sensitive fluorogenic substrates for glutathione S-transferase (GST) and application for activity imaging in living cells. J Am Chem Soc. 2008 130(44):14533-43. doi: 10.1021/ja802423n.
Yogo T, Urano Y, Mizushima A, Sunahara H, Inoue T, Hirose K, Iino M, Kikuchi K, Nagano T: Selective photoinactivation of protein function through environment-sensitive switching of singlet oxygen generation by photosensitizer. Proc Natl Acad Sci U S A. 2008 105(1):28-32. doi: 10.1073/pnas.0611717105.
2007
Kobayashi H, Koyama Y, Barrett T, Hama Y, Regino CA, Shin IS, Jang BS, Le N, Paik CH, Choyke PL, Urano Y: Multimodal nanoprobes for radionuclide and five-color near-infrared optical lymphatic imaging. ACS Nano. 2007 1(4):258-64. doi: 10.1021/nn700062z.
Hama Y, Koyama Y, Urano Y, Choyke PL, Kobayashi H: Simultaneous two-color spectral fluorescence lymphangiography with near infrared quantum dots to map two lymphatic flows from the breast and the upper extremity. Breast Cancer Res Treat. 2007 103(1):23-8. doi: 10.1007/s10549-006-9347-0.
Matsumoto T, Urano Y, Shoda T, Kojima H, Nagano T: A thiol-reactive fluorescence probe based on donor-excited photoinduced electron transfer: Key role of ortho substitution. Org Lett. 2007 9(17):3375-7. doi: 10.1021/ol071352e.
Koyama Y, Hama Y, Urano Y, Nguyen DM, Choyke PL, Kobayashi H: Spectral fluorescence molecular imaging of lung metastases targeting HER2/neu. Clin Cancer Res. 2007 13(10):2936-45. doi: 10.1158/1078-0432.CCR-06-2240.
Hama Y, Urano Y, Koyama Y, Choyke PL, Kobayashi H: Activatable fluorescent molecular imaging of peritoneal metastases following pretargeting with a biotinylated monoclonal antibody. Cancer Res. 2007 67(8):3809-17. doi: 10.1158/0008-5472.CAN-06-3794.
Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H: A target-cell specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate. Cancer Res. 2007 67(6):2791-9. doi: 10.1158/0008-5472.CAN-06-3315.
Koide Y, Urano Y, Kenmoku S, Kojima H, Nagano T: Design and synthesis of fluorescent probes for selective detection of highly reactive oxygen species in mitochondria of living cells. J Am Chem Soc. 2007 129(34):10324-5. doi: 10.1021/ja073220m.
Kenmoku S, Urano Y, Kojima H, Nagano T: Development of a highly specific, rhodamine-based fluorescence probe for hypochloroous acid and its application to real-time imaging of phagocytosis. J Am Chem Soc. 2007 129(23):7313-8. doi: 10.1021/ja068740g.
Sunahara H, Urano Y, Kojima H, Nagano T: Design and synthesis of a library of BODIPY-based environmental polarity sensors utilizing photoinduced electron transfer-controlled fluorescence ON/OFF switching. J Am Chem Soc. 2007 129(17):5597-604. doi: 10.1021/ja068551y.
Komatsu K, Urano Y, Kojima H, Nagano T: Development of an iminocoumarin-based zinc sensor suitable for ratiometric fluorescence imaging of neuronal zinc. J Am Chem Soc. 2007 129(44):13447-54. doi: 10.1021/ja072432g.
Kobayashi T, Urano Y, Kamiya M, Ueno T, Kojima H, Nagano T: Highly activatable and rapidly releasable caged fluorescein derivatives. J Am Chem Soc. 2007 129(21):6696-7. doi: 10.1021/ja070376d.
Kamiya M, Kobayashi H, Hama Y, Koyama Y, Bernardo M, Nagano T, Choyke PL, Urano Y: An enzymatically activated fluorescence probe for targeted tumor imaging. J Am Chem Soc. 2007 129(13):3918-29. doi: 10.1021/ja067710a.
2006
Hama Y, Urano Y, Koyama Y, Choyke PL, Kobayashi H: Targeted optical imaging of cancer cells using lectin-binding BODIPY conjugated avidin. Biochem Biophys Res Commun. 2006 348(3):807-13. doi: 10.1016/j.bbrc.2006.07.169.
Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik R, Krishna M, Choyke PL, Kobayashi H: In vivo spectral fluorescence molecular imaging of submillimeter peritoneal cancer implants using a lectin-tageted optical agent. Neoplasia. 2006 8(7):607-12. doi: 10.1593/neo.06268.
Hama Y, Urano Y, Koyama Y, Bernardo M, Choyke PL, Kobayashi H: A comparison of the emission efficiency of four common green fluorescence dyes after internalization into cancer cells. Bioconjug Chem. 2006 17(6):1426-31. doi: 10.1021/bc0601626.
Kiyose K, Kojima H, Urano Y, Nagano T: Development of a ratiometric fluorescent zinc ion probe in near-infrared region, based on tricarbocyanine chromophore. J Am Chem Soc. 2006 128(20):6548-9. doi: 10.1021/ja060399c.
Komatsu T, Kikuchi K, Takakusa H, Hanaoka K, Ueno T, Kamiya M, Urano Y, Nagano T: Design and synthesis of an enzyme activity-based labeling molecule with fluorescence spectral change. J Am Chem Soc. 2006 128(50):15946-7. doi: 10.1021/ja0657307.
Mineno T, Ueno T, Urano Y, Kojima H, Nagano T: Creation of superior carboxyfluorescein dyes by blocking donor-excited photoinduced electron transfer.Org Lett. 2006 8(26):5963-6. doi: 10.1021/ol0623926.
Gabe Y, Ueno T, Urano Y, Kojima H, Nagano T: Tunable design strategy for fluorescence probes based on 4-Substituted BODIPY chromophore: Improvement of highly sensitive fluorescence probe for Nitric Oxide. Anal Bioanal Chem. 2006 386(3):621-6. doi: 10.1007/s00216-006-0587-y.
Terai T, Kikuchi K, Iwasawa S, Kawabe T, Hirata Y, Urano Y, Nagano T: Modulation of luminescence intensity of Lanthanide complexes by photoinduced electron transfer and its application to a long-lived protease robe. J Am Chem Soc. 2006 128(21):6938-46. doi: 10.1021/ja060729t.
Ueno T, Urano Y, Kojima H, Nagano T: Mechanism-based molecular design of highly selective fluorescence probes for nitrative stress. J Am Chem Soc. 2006 128(33):10640-1. doi: 10.1021/ja061972v.
2005
Tomizawa S, Imai H, Tsukada S, Simizu T, Honda F, Nakamura M, Nagano T, Urano Y, Matsuoka Y, Fukasaku N, Saito N: The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia. Neurosci Res. 2005 53(3):304-13. doi: 10.1016/j.neures.2005.08.002.
Hasobe T, Hattori S, Kamat PV, Urano Y, Umezawa N, Nagano T, Fukuzumi S: Organization of supramolecular assemblies of fullerene, porphyrin and fluorescein dye derivatives on TiO2 nanoparticles for light energy conversion. Chem. Phys. 2005 319:243-252. doi: 10.1016/j.chemphys.2005.06.035.
Hattori S, Ohkubo K, Urano Y, Sunahara H, Nagano T, Wada Y, Tkachenko NV, Lemmetyinen H, Fukuzumi S: Charge separation in a nonfluorescent donor-acceptor dyad derived from Boron dipyrromethene dye, leading to photocurrent generation. J Phys Chem B. 2005 109(32):15368-75. doi: 10.1021/jp050952x.
Kadowaki H, Nishitoh H, Urano F, Sadamitsu C, Matsuzawa A, Takeda K, Masutani H, Yodoi J, Urano Y, Nagano T, Ichijo H: Amyloid beta induces neuronal cell death through ROS-mediated ASK1 activation. Cell Death Differ. 2005 12(1):19-24. doi: 10.1038/sj.cdd.4401528.
Kawabata E, Kikuchi K, Urano Y, Kojima H, Odani A, Nagano T: Design and synthesis of zinc-selective chelators for extracellular applications. J Am Chem Soc. 2005 127(3):818-9. doi: 10.1021/ja044697q.
Komatsu K, Kikuchi K, Kojima H, Urano Y, Nagano T: Selective zinc sensor molecules with various affinities for Zn2+, revealing dynamics and regional distribution of synaptically released Zn2+ in hippocampal slices. J Am Chem Soc. 2005 127(29):10197-204. doi: 10.1021/ja050301e.
Sasaki E, Kojima H, Nishimatsu H, Urano Y, Kikuchi K, Hirata Y, Nagano T: Highly sensitive near-infrared fluorescence probes for nitric oxide and their application to isolated organs. J Am Chem Soc. 2005 127(11):3684-5. doi: 10.1021/ja042967z.
Kamiya M, Urano Y, Ebata N, Yamamoto M, Kosuge J, Nagano T: Extension of the applicable range of fluorescein: A novel fluorescein-based probe for Western blot analysis. Angew Chem Int Ed Engl. 2005 44(34):5439-41. doi: 10.1002/anie.200501542.
Yogo T, Urano Y, Ishitsuka Y, Maniwa F, Nagano T: Highly efficient and photostable photosensitizer based on BODIPY chromophore. J Am Chem Soc. 2005 127(35):12162-3. doi: 10.1021/ja0528533.
Urano Y, Kamiya M, Kanda K, Ueno T, Hirose K, Nagano T: Evolution of fluorescein as a platform for finely tunable fluorescence probes. J Am Chem Soc. 2005 127(13):4888-94. doi: 10.1021/ja043919h.
2004
Hattori S, Hasobe T, Ohkubo K, Urano Y, Umezawa N, Nagano T, Wada Y, Yanagida S, Fukuzumi S: Enhanced energy and quantum efficiencies of a nanocrystalline photoelectrochemical cell sensitized with a donor-acceptor dyad derived from fluorescein. J. Phys. Chem. 2004 B 108:15200-15205. doi: 10.1021/jp047656p.
Hanaoka K, Kikuchi K, Kojima H, Urano Y, Nagano T: Development of a zinc ion-selective luminescent lanthanide chemosensor for biological applications. J Am Chem Soc. 2004 126(39):12470-6. doi: 10.1021/ja0469333.
Koutaka H, Kosuge J, Fukasaku N, Hirano T, Kikuchi K, Urano Y, Kojima H, Nagano T: A novel fluorescent probe for zinc ion based on BODIPY chromophore. Chem Pharm Bull (Tokyo). 2004 52(6):700-3. doi: 10.1248/cpb.52.700.
Ueno T, Urano Y, Setsukinai K, Takakusa H, Kojima H, Kikuchi K, Ohkubo K, Fukuzumi S, Nagano T: Rational principles for modulating fluorescence properties of fluorescein. J Am Chem Soc. 2004 126(43):14079-85. doi: 10.1021/ja048241k.
Gabe Y, Urano Y, Kikuchi K, Kojima H, Nagano T: Highly sensitive fluorescence probes for nitric oxide based on boron dipyrromethene chromophore: Rational design of potentially useful bioimaging fluorescence probe. J Am Chem Soc. 2004 126(10):3357-67. doi: 10.1021/ja037944j.
Kenmoku S, Urano Y, Kanda K, Kojima H, Kikuchi K, Nagano T: Rational design of novel photoinduced electron transfer type fluorescent probes for sodium cation. Tetrahedron 2004 60:11067-11073. doi: 10.1016/j.tet.2004.06.108.
2003
Hanaoka K, Kikuchi K, Kojima H, Urano Y, Nagano T: Selective detection of zinc ions with novel luminescent lanthanide probes. Angew Chem Int Ed Engl. 2003 42(26):2996-9. doi: 10.1002/anie.200351122.
Shoda T, Kikuchi K, Kojima H, Urano Y, Komatsu H, Suzuki K, Nagano T: Development of selective, visible light-excitable, fluorescent magnesium probes with a novel fluorescence switching mechanism. Analyst. 2003 128(6):719-23. doi: 10.1039/B212835G.
Setsukinai K, Urano Y, Kakinuma K, Majima HJ, Nagano T: Development of novel fluorescence probes that can reliably detect reactive oxygen species and distinguish specific species. J Biol Chem. 2003 278(5):3170-5. doi:10.1074/jbc.M209264200.
Miura T, Urano Y, Tanaka K, Nagano T, Ohkubo K, Fukuzumi S: Rational design principle for modulating fluorescence properties of fluorescein-based probes by photoinduced electron transfer. J Am Chem Soc. 2003 125(28):8666-71. doi: 10.1021/ja035282s.
2002
Takakusa H, Kikuchi K, Urano Y, Sakamoto S, Yamaguchi K, Nagano T: Design and synthesis of an enzyme-cleavable sensor molecule for phosphodiesterase activity based on fluorescence resonance energy transfer. J. Am. Chem. Soc. 2002 124(8):1653-7. doi: 10.1021/ja011251q.
Hanaoka K, Kikuchi K, Urano Y, Narazaki M, Yokawa T, Sakamoto S, Yamaguchi K, Nagano T: Design and synthesis of a novel magnetic resonance imaging contrast agent for selective sensing of zinc ion. Chem. Biol. 2002 9(9):1027-32. doi: 10.1016/S1074-5521(02)00216-8.
Makino Y, Urano Y, Nagano T: Impurity profiling of ephedrines in methamphetamine by high-performance liquid chromatography. J. Chromatography A 2002 947(1):151-4. doi: 10.1016/S0021-9673(01)01594-1.
Nishida M, Schey KL, Takagahara S, Kontani K, Katada T, Urano Y, Nagano T, Nagao T, Kurose H: Activation mechanism of Gi and Go as a redox sensor. J. Biol. Chem. 2002 277(11):9036-42. doi: 10.1074/jbc.M107392200.
Suzuki N, Kojima H, Urano Y, Kikuchi K, Hirata Y, Nagano T: Orthogonality of Calcium concentration and ability of 4,5-diaminofluorescein (DAF-2) to detect NO. J. Biol. Chem. 2002 277(1):47-9 doi: 10.1074/jbc.M108195200.
Mizukami S, Nagano T, Urano Y, Odani A, Kikuchi K: A fluorescent anion sensor that works in neutral aqueous solution for bioanalytical application. J. Am. Chem. Soc. 2002 124(15):3920-5. doi: 10.1021/ja0175643.
Hirano T, Kikuchi K, Urano Y, Nagano T: Improved fluorescent probes for zinc, ZnAFs, suitable for biological applications. J. Am. Chem. Soc. 2002 124(23):6555-62. doi: 10.1021/ja025567p.
Maruyama S, Kikuchi K, Hirano T, Urano Y, Nagano T: A novel, cell-permeable, fluorescent probe for ratiometric imaging of zinc ion. J. Am. Chem. Soc. 2002 124(36):10650-1. doi: 10.1021/ja026442n.
2001
Kojima H, Hirotani M, Nakatsubo N, Kikuchi K, Urano Y, Higuchi T, Hirata Y, Nagano T: Bioimaging of nitric oxide with fluorescent indicators based on rhodamine chromophore. Anal. Chem. 2001 73(9):1967-73. doi: 10.1021/ac001136i.
Takakusa H, Kikuchi K, Urano Y, Higuchi T, Nagano T: Intramolecular fluorescence resonance energy transfer system with coumarin donor included in beta-cyclodextrin. Anal. Chem. 2001 73(5):939-42. doi: 10.1021/ac001016a.
Hanaoka K, Kikuchi K, Urano Y, Nagano T: Selective sensing of zinc ion with a novel magnetic resonance imaging contrast agent. J. Chem. Soc., Perkin Transaction 2001 2: 1840-1843. doi: 10.1039/B100994J.
Park JM, Higuchi T, Kikuchi K, Urano Y, Hori H, Nishino T, Aoki J, Inoue K, Nagano T: Selective inhibition of human inducible nitric oxide synthase by S-alkyl-L-isothiocitrulline-containing dipeptides. British J. Pharmacol. 2001 132(8):1876-82. doi: 10.1038/sj.bjp.0704023.
Wada M, Ito S, Uno H, Murashima T, Ono N, Urano T, Urano Y: Synthesis and optical properties of a new class of pyrromethene-BF2 complex fused with rigid bicycle rings and benzo derivatives. Tetrahedron Lett. 2001 42(38):6711-6713.
Tanaka K, Miura T, Umezawa N, Urano Y, Kikuchi K, Higuchi T, Nagano T: Rational design of fluorescein-based fluorescence probes: Mechanism-based design of a maximum fluorescence probe for singlet oxygen. J. Am. Chem. Soc. 2001 123(11):2530-6. doi: 10.1021/ja0035708.
2000
Kawanishi Y, Kikuchi K, Takakusa H, Mizukami S, Urano Y, Higuchi T, Nagano T: Design and synthesis of intramolecular resonance energy transfer probes for use in aqueous solution. Angew. Chem. Int. Ed. 2000 39(19):3438-3440. doi: 10.1002/1521-3773(20001002)39:19<3438::AID-ANIE3438>3.0.CO;2-T.
Hirano T, Kikuchi K, Urano Y, Higuchi T, Nagano T: Novel zinc fluorescent probes excitable with visible light for biological applications. Angew. Chem. Int. Ed. 2000 39(6):1052-1054. doi: 10.1002/(SICI)1521-3773(20000317)39:6<1052::AID-ANIE1052>3.0.CO;2-5.
Koresawa M, Kikuchi K, Mizukami S, Kojima H, Urano Y, Higuchi T, Nagano T: Development of time-resolved fluorometric detection system using diffusion-enhanced energy transfer. Anal. Chem. 2000 72(20):4904-7 doi: 10.1021/ac000356t.
Tamura M, Urano Y, Kikuchi K, Higuchi T, Hirobe M, Nagano T: Superoxide dismutase activity of Iron(II)-TPEN complex and its derivatives. Chem. Pharm. Bull. 12000 48(10):1514-8. doi: 10.1248/cpb.48.1514.
Ohno T, Suzuki N, Dokoh T, Urano Y, Kikuchi K, Hirobe M, Higuchi T, Nagano T: Remarkable axial thiolate ligand effects on the oxidation of hydrocarbons by active intermediate of iron porphyrin and cytochrome P450. J. Inorg. Biochem. 2000 82(1-4):123-5. doi: 10.1016/S0162-0134(00)00141-0.
Kojima H, Hirotani M, Urano Y, Kikuchi K, Higuchi T, Nagano T: Fluorescent indicators for nitric oxide based on rhodamine chromophore. Tetrahedron Lett. 2000 38(21):3209-3212. doi: 10.1002/(SICI)1521-3773(19991102)38:21<3209::AID-ANIE3209>3.0.CO;2-6.
Dokoh T, Suzuki N, Higuchi T, Urano Y, Kikuchi K, Nagano T: A new thioether-ligated iron porphyrin as a model of a protonated form of P450 active site. J. Inorg. Biochem. 2000 82(1-4):127-32. doi: 10.1016/S0162-0134(00)00135-5.
Tamura M, Urano Y, Kikuchi K, Higuchi T, Hirobe M, Nagano T: Synthesis and superoxide dismutase activity of novel iron complexes. J. Organomet. Chem. 2000 611(1-2): 586-592. doi: 10.1016/S0022-328X(00)00394-6.
Setsukinai K, Urano Y, Kikuchi K, Higuchi T, Nagano T: Fluorescence switching by O-dearylation of 7-aryloxycoumarines: Development of novel fluorescence probes to detect reactive oxygen species with high selectivity. Perkin Transaction 2000 2: 2453-2457. doi: 10.1039/B006449L.
Suzuki N, Higuchi T, Urano Y, Kikuchi K, Uchida T, Mukai M, Kitagawa T, Nagano T: First synthetic NO-heme-thiolate complex relevant to nitric oxide synthase and cytochrome P450nor. J. Am. Chem. Soc. 2000 122(48): 12059-12062. doi: 10.1021/ja005670j.
Hirano T, Kikuchi K, Urano Y, Higuchi T, Nagano T: Highly zinc-selective fluorescent sensor molecules suitable for biological applications. J. Am. Chem. Soc. 2000 122(49): 12399-12400. doi: 10.1021/ja002467f.
1999
Mizukami S, Kikuchi K, Higuchi T, Urano Y, Mashima T, Tsuruo T, Nagano T: Imaging of caspase-3 activation in HeLa cells stimulated with etoposide using a novel fluorescent probe. FEBS Lett. 1999 453(3):356-60. doi: 10.1016/S0014-5793(99)00755-3.
Yamaguchi K, Makino Y, Urano Y, Nagano T: Absolute configuration of cathine. Anal. Sci. 1999 15(10): 1039-1040. doi: 10.2116/analsci.15.1039.
Kobayashi N, Higuchi T, Urano Y, Kikuchi K, Hirobe M, Nagano T: Dipeptides containing L-arginine analogs: new isozyme-selective inhibitors of nitric oxide synthase. Biol. Pharm. Bull. 1999 22: 936-940 doi: 10.1248/bpb.22.936.
Kojima H, Urano Y, Kikuchi K, Higuchi T, Nagano T: Fluorescent indicators for imaging nitric oxide production. Angew. Chem. Int. Ed. 1999 38(21):3209-3212. doi: 10.1002/(SICI)1521-3773(19991102)38:21<3209::AID-ANIE3209>3.0.CO;2-6.
Suzuki N, Higuchi T, Urano Y, Kikuchi K, Uekusa H, Ohashi Y, Uchida T, Kitagawa T, Nagano T: Novel iron porphyrin-alkanethiolate complex with intramolecular NH...S hydrogen bond: synthesis, spectroscopy and reactivity. J. Am. Chem. Soc. 1999 121(49): 11571-11572. doi: 10.1021/ja992511v.
Umezawa N, Tanaka K, Urano Y, Kikuchi K, Higuchi T, Nagano T: Novel fluorescent probes for singlet oxygen. Angew. Chem. Int. Ed. 1999 38(19):2899-2901. doi: 10.1002/(SICI)1521-3773(19991004)38:19<2899::AID-ANIE2899> 3.0.CO;2-M.
1998
Kojima H, Nakatsubo N, Kikuchi K, Urano Y, Higuchi T, Tanaka J, Kudo Y, Nagano T: Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA. NeuroReport 1998 9(15):3345-8. doi: 10.1097/00001756-199810260-00001.
Nakatsubo N, Kojima H, Sakurai K, Kikuchi K, Nagoshi H, Hirata Y, Akaike T, Maeda H, Urano Y, Higuchi T, Nagano T: Improved nitric oxide detection using 2,3-diaminonaphthalene and its application to the evaluation of novel nitric oxide synthase inhibitors. Biol. Pharm. Bull.1998 21(12):1247-50. doi: 10.1248/bpb.21.1247.
Nakagawa H, Higuchi T, Kikuchi K, Urano Y, Nagano T: Selective deoxygenation of heteroaromatic N-oxides with olefins catalyzed by Ruthenium porphyrin. Chem. Pharm. Bull. 1998 46: 1656-1657. doi: 10.1248/cpb.46.1656.
-1997
Urano Y, Higuchi T, Hirobe M, Nagano T: Pronounced axial thiolate ligand effect on the reactivity of high-valent oxo-iron porphyrin intermediate. J. Am. Chem. Soc. 1997 119(49): 12008-12009. doi: 10.1021/ja972892h.
Urano Y, Higuchi T, Hirobe M: Substrate-dependent changes of the oxidative O-dealkylation mechanism of several chemical and biological oxidizing systems. J. Chem. Soc. Perkin Trans. 1996 2: 1169-1173. doi: 10.1039/P29960001169.
Aihara K, Urano Y, Higuchi T, Hirobe M: Mechanistic studies of selective catechol formation from o-methoxyphenols using a Copper(II)-ascorbic acid-dioxygen system. J. Chem. Soc. Perkin Trans. 1993 2: 2165-2170. doi: 10.1039/P29930002165.
Urano Y, Higuchi T, Hirobe M: Unusual substituent effects in the hydroxylation of phenols by a Cu2+-ascorbic acid-O2 system, g-radiolysis, and microsomes. Biochem. Biophys. Res. Commun. 1993 192(2): 568-574. doi: 10.1006/bbrc.1993.1453.